| Expression pattern: |
DN |
| Associated gene: |
Wnt3a, Wnt5a, beta-catenin, caspase 3, p53, PARP |
| Associated microRNA: |
miR-17-5p, miR-17a-5p |
| Biological function: |
circ-ITCH protects H2O2-treated myocardial cells by increasing cell viability and ATP concentration and suppressing apoptosis. |
| Molecular mechanism: |
circ-ITCH absorbs/targets miR-17-5p and negatively regulates Wnt/beta-catenin signalling pathway factors, thereby suppressing apoptosis-related proteins in H2O2-treated myocardial cells. |
| Biological pathway or process: |
Wnt/beta-catenin (inhibits); apoptosis (inhibits); ceRNA regulation (other); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot |
| Clinical significance: |
- |
| Description: |
circ-ITCH is down-regulated in H2O2-induced H9c2 myocardial injury. Its overexpression protects myocardial cells by improving viability and ATP levels and suppressing apoptosis, partly through targeting miR-17-5p and negatively regulating Wnt/beta-catenin signalling. |
| Confidence score: |
0.5554 |