| Expression pattern: |
DN |
| Associated gene: |
c-Met, WNT, PI3K, TGF-beta |
| Associated microRNA: |
miR-34b-5p, miR-29a-5p, miR-223a-3p, miR-876-3p, miR-215-3p |
| Biological function: |
Down-regulated in bone marrow-derived exosomes from ET patients; predicted to regulate cell proliferation and apoptosis through miRNA-mediated targeting of functional genes and signaling pathways. |
| Molecular mechanism: |
Predicted circRNA-miRNA-mRNA ceRNA network in which circASXL1 targets miR-34b-5p and other miRNAs, with downstream regulation of c-Met, WNT, PI3K and TGF-beta signaling components. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (other); apoptosis (other); Wnt/beta-catenin (other); PI3K/AKT (other); TGF-beta/SMAD (other); other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
Potential basis for circRNA-based ET diagnosis and treatment. |
| Description: |
hsa_circ_0001136/circASXL1 is down-regulated in bone marrow-derived exosomes from ET patients and was validated by Sanger sequencing, divergent-primer RT-PCR, and qRT-PCR. Bioinformatics analysis predicts that circASXL1 may regulate ET-related proliferation and apoptosis programs through miR-34b-5p and other miRNAs targeting c-Met, WNT, PI3K, and TGF-beta pathways. |
| Confidence score: |
0.5841 |