| Expression pattern: |
UP |
| Associated gene: |
multidrug resistance-associated protein 1, P-glycoprotein, MRP1, P-gp |
| Associated microRNA: |
- |
| Biological function: |
Exosomal hsa_circ_103801 promotes cisplatin resistance in osteosarcoma cells, reduces sensitivity to CDDP, inhibits apoptosis, and increases resistance-related proteins MRP1 and P-gp. |
| Molecular mechanism: |
CDDP-resistant osteosarcoma cell-derived exosomes transfer hsa_circ_103801 to recipient chemosensitive osteosarcoma cells, thereby enhancing CDDP resistance and increasing MRP1 and P-gp expression while decreasing apoptosis. |
| Biological pathway or process: |
chemoresistance (promotes); drug resistance (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Survival Analysis; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot |
| Clinical significance: |
Serum exosomal hsa_circ_103801 may serve as a prognostic biomarker for OS; high expression is associated with shorter survival time. |
| Description: |
hsa_circ_103801 is upregulated in serum exosomes from osteosarcoma patients and in cisplatin-resistant osteosarcoma cells/exosomes. CDDP-resistant cell-derived exosomes transfer hsa_circ_103801 to recipient MG63 and U2OS cells, promoting cisplatin resistance, inhibiting apoptosis, and increasing MRP1 and P-gp expression. High serum exosomal hsa_circ_103801 expression is associated with shorter survival, suggesting prognostic value and potential as a target for overcoming OS chemoresistance. |
| Confidence score: |
0.6148 |