| Expression pattern: |
UP |
| Associated gene: |
NPSR1, PGPEP1 |
| Associated microRNA: |
miR-1182 |
| Biological function: |
Promotes pancreatic cancer cell proliferation, colony formation, migration, invasion and tumorigenesis; inhibits apoptosis and G0/G1 cell-cycle arrest. |
| Molecular mechanism: |
circ-0050102 acts as a competing endogenous RNA by sponging miR-1182, reducing miR-1182 expression and relieving miR-1182-mediated suppression of NPSR1. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); cell cycle (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; FISH / smFISH; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; In Vivo Animal Model; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ-0050102 expression was potentially correlated with the malignancy or severity of pancreatic cancer and may be a promising therapeutic target. |
| Description: |
hsa_circ_0050102/circ-0050102 is up-regulated in pancreatic cancer tissues and PDAC cell lines. It promotes pancreatic cancer proliferation, migration, invasion and xenograft tumor growth while suppressing apoptosis and cell-cycle arrest. Mechanistically, it functions as a ceRNA by sponging miR-1182 to increase NPSR1 expression, suggesting the circ-0050102/miR-1182/NPSR1 axis as a potential therapeutic strategy. |
| Confidence score: |
0.8071 |