circRNA basic information
circBase ID: hsa_circ_0002483
Name: hsa_circ_PTK2
Synonym: circ_0002483
Host Gene: PTK2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0018874
MONDO name: acute myeloid leukemia
Disease details: acute myeloid leukemia
Disease DO ID:
9119
Disease MeSH ID:
D015470
Disease NCIt ID:
C3171
Disease ICD11 ID:
-
Disease OMIM ID:
601626
Species: Human
Species details: Homo sapiens
Tissue specimen:

bone marrow tissues; bone marrow samples

Cell lines:

HL-60; AML-193; HS-5

In vivo animal model:

-

circRNA-disease information
Expression pattern:
UP
Associated gene: MYC
Associated microRNA: miR-758-3p
Biological function: promotes AML progression, enhances cell proliferation, promotes cell cycle progression, and inhibits apoptosis
Molecular mechanism: circ_0002483 targets miR-758-3p and indirectly regulates MYC through the circ_0002483/miR-758-3p/MYC axis
Biological pathway or process:

proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis

Clinical significance:

circ_0002483 is highly expressed in bone marrow tissues from AML patients and may provide a new target for AML treatment

Description:

Hsa_circ_0002483/circ_0002483 is up-regulated in AML bone marrow samples and AML cell lines. Functionally, circ_0002483 promotes AML cell proliferation and cell cycle progression while inhibiting apoptosis. Mechanistically, it regulates the miR-758-3p/MYC axis, suggesting a potential therapeutic target in AML.

Confidence score:

0.8111

Other information
Title:

Hsa_circ_0002483 regulates miR-758-3p/MYC axis to promote acute myeloid leukemia progression.

Journal: Hematological oncology
Published: 2021
PubMed ID: 33283885
Study type:

combined biological and clinical study

Data availability: The data sets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -