| Expression pattern: |
UP |
| Associated gene: |
Smad7 |
| Associated microRNA: |
miR-424 |
| Biological function: |
Promotes proliferation, migration, invasion, EMT, tumor growth and metastasis of colorectal cancer cells; knockdown reduces migration and invasion. |
| Molecular mechanism: |
circTBL1XR1 acts as a ceRNA/sponge for miR-424, inhibits miR-424, and increases Smad7 expression to promote malignant progression of colorectal cancer. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); MAPK (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Luciferase Reporter Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circTBL1XR1 is highly expressed in colorectal cancer and higher in patients with metastasis; it may be a therapeutic target for colon cancer. |
| Description: |
circTBL1XR1 is up-regulated in colorectal cancer tissues and cell lines and is associated with metastatic disease. It promotes colorectal cancer cell proliferation, migration, invasion, EMT and tumor growth by sponging miR-424 and relieving miR-424-mediated repression of Smad7. The study proposes the circTBL1XR1/miR-424/Smad7/MAPK8 pathway as a potential therapeutic target in colon cancer. |
| Confidence score: |
0.7126 |