circRNA basic information
circBase ID: hsa_circ_0009034
Name: hsa_circ_MAP3K5
Synonym: CircMAP3K5
Host Gene: -
Genomic location(hg19): chr6:136990420-137019820:-
Genomic location(hg38): chr6:136669282-136698682:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005043
MONDO name: hyperplasia
Disease details: intimal hyperplasia
Disease DO ID:
 -
Disease MeSH ID:
 D006965
Disease NCIt ID:
 C3113
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

human coronary arteries; mouse femoral arteries; mouse tissues including fat, liver, kidney, brain, lung, artery and spleen
Cell lines:

HCASMCs; MASMC; HEK-293T
In vivo animal model:

genetically engineered animal model; other disease animal model
circRNA-disease information
Expression pattern:
DN
Associated gene: TET2 / Tet2 / Ago2
Associated microRNA: miR-22-3p / miR-22
Biological function: CircMAP3K5 inhibits SMC proliferation, promotes VSMC/SMC differentiation, inhibits SMC dedifferentiation, and attenuates intimal hyperplasia after vascular injury.
Molecular mechanism: CircMAP3K5 acts as a cytoplasmic competing endogenous RNA/miRNA sponge that sequesters miR-22-3p, relieving miR-22-3p-mediated inhibition of TET2 and promoting TET2-mediated VSMC differentiation.
Biological pathway or process:

proliferation (inhibits); ceRNA regulation (promotes); other pathway/process (promotes)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; In Vivo Animal Model; H&E Staining; IF (Immunofluorescence); Western Blot; Bioinformatics Analysis
Clinical significance:

CircMAP3K5 was significantly decreased in human coronary arteries from patients with CHD and targeting the CircMAP3K5/miR-22-3p/TET2 axis may provide a potential therapeutic strategy for intimal hyperplasia, restenosis and atherosclerosis.
Description:

CircMAP3K5 is down-regulated in CHD arteries and in wire-injury-associated intimal hyperplasia. It functions as a cytoplasmic miR-22-3p sponge, increasing TET2 expression to promote VSMC differentiation and suppress SMC proliferation, thereby reducing neointima formation. The CircMAP3K5/miR-22-3p/TET2 axis is proposed as a therapeutic target for intimal hyperplasia-related vascular disease.
Confidence score:

0.8573
Other information
Title:

Circular RNA CircMAP3K5 Acts as a MicroRNA-22-3p Sponge to Promote Resolution of Intimal Hyperplasia Via TET2-Mediated Smooth Muscle Cell Differentiation.

Journal: Circulation
Published: 2021
PubMed ID: 33207953
Study type:

combined biological and clinical study
Data availability: Available from the corresponding author on reasonable request
Code availability: -