| Expression pattern: |
UP |
| Associated gene: |
HDAC5, Aggf1 |
| Associated microRNA: |
miR-331-3p |
| Biological function: |
Facilitates VSMC proliferation and migration and promotes phenotypic modulation toward a proliferative synthetic phenotype; circSFMBT2 knockdown suppresses VSMC proliferation and migration and enhances contractile marker expression. |
| Molecular mechanism: |
circSFMBT2 acts as a competing endogenous RNA sponge for miR-331-3p, up-regulates HDAC5, and regulates Aggf1 transcription/activity through the circSFMBT2/miR-331-3p/HDAC5/Aggf1 axis. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); cell cycle (promotes); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RNA Pull-Down; ChIP / ChIP-seq; Co-IP; Luciferase Reporter Assay; Transfection; MTT; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Potential therapeutic target for treating restenosis and proliferative vascular diseases. |
| Description: |
circSFMBT2 (hsa_circ_0000212) is up-regulated in human restenosis/neointimal tissue and PDGF-BB-treated human VSMCs. It promotes VSMC proliferation, migration, cell-cycle progression, and phenotypic modulation by sponging miR-331-3p and increasing HDAC5, which regulates Aggf1. The study suggests circSFMBT2 knockdown as a potential therapeutic strategy for restenosis and proliferative vascular diseases. |
| Confidence score: |
0.8338 |