circRNA basic information
circBase ID: hsa_circ_0005224
Name: hsa_circ_TGFBR2
Synonym: circTGFBR2
Host Gene: TGFBR2
Genomic location(hg19): chr3:30686238-30691952:+
Genomic location(hg38): chr3:30644746-30650460:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0015459
MONDO name: nasopharyngeal carcinoma
Disease details: nasopharyngeal carcinoma
Disease DO ID:
9261
Disease MeSH ID:
D00007727; D000077274
Disease NCIt ID:
C3871
Disease ICD11 ID:
1883313543
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

NPC tissue samples; adjacent normal tissue samples; normal nasopharyngeal mucosal tissues; lung tissue samples

Cell lines:

HNE1; HONE1; 5-8F; 6-10B; C666-1; NP69; HEK-293; HEK-293T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
DN
Associated gene: TGFBR2, PI3K/Akt pathway
Associated microRNA: miR-107
Biological function: Suppresses NPC cell proliferation, migration, invasion, EMT, tumor growth, and lung metastasis; promotes apoptosis; acts as a tumor suppressive factor.
Molecular mechanism: circTGFBR2 acts as a ceRNA/miR-107 sponge, upregulates TGFBR2 expression, inhibits PI3K/Akt pathway activity, and suppresses NPC progression.
Biological pathway or process:

PI3K/AKT (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); apoptosis (promotes); ceRNA regulation (other)

Detected method:
Q
H
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; ISH (In Situ Hybridization); FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; IHC (Immunohistochemistry); IF (Immunofluorescence); Western Blot; In Vivo Animal Model; H&E Staining; Cohort Study; Survival Analysis; Bioinformatics Analysis

Clinical significance:

circTGFBR2 overexpression correlated with favorable prognosis in NPC; low circTGFBR2 levels were associated with worse overall survival and metastasis-free survival; circTGFBR2 may serve as a promising diagnostic biomarker and therapeutic target.

Description:

circTGFBR2 is a TGFBR2-derived circRNA downregulated in NPC tissues and cells. It functions as a tumor suppressor by sponging miR-107, increasing TGFBR2 expression, inhibiting PI3K/Akt activity and EMT, and suppressing proliferation, migration, invasion, tumor growth, and metastasis. Higher circTGFBR2 expression is associated with favorable NPC prognosis.

Confidence score:

0.8648

Other information
Title:

Circular RNA TGFBR2 acts as a ceRNA to suppress nasopharyngeal carcinoma progression by sponging miR-107.

Journal: Cancer letters
Published: 2021
PubMed ID: 33160003
Study type:

combined biological and clinical study

Data availability: GSE70970; GSE118720; Supplementary data related to this article can be found at https ://doi.org/10.1016/j.canlet.2020.11.001; All data that support the findings of this study are available from the corresponding authors upon reasonable request.
Code availability: -