| Expression pattern: |
UP |
| Associated gene: |
FOXM1 |
| Associated microRNA: |
miR-873-5p |
| Biological function: |
circ_0031250 promotes NSCLC cell proliferation, migration, invasion and in vivo tumor growth, inhibits apoptosis, and counteracts the anti-cancer effects of genistein. |
| Molecular mechanism: |
circ_0031250 acts through a ceRNA mechanism by sponging miR-873-5p to regulate FOXM1 expression; genistein reduces circ_0031250, thereby decreasing FOXM1 and suppressing NSCLC progression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes); drug resistance (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
High circ_0031250 expression was associated with tumor size, TNM stages, lymphatic metastasis, and worse outcomes in NSCLC patients. |
| Description: |
hsa_circ_0031250/circ_0031250 is up-regulated in NSCLC tissues and cell lines and is reduced by genistein treatment. It functions as an oncogenic circRNA by sponging miR-873-5p to regulate FOXM1, thereby promoting proliferation, migration, invasion and tumor growth while inhibiting apoptosis. Knockdown of circ_0031250 enhances the anti-NSCLC effects of genistein in vitro and in a cell line-derived xenograft model. |
| Confidence score: |
0.78 |