circRNA basic information
circBase ID: hsa_circ_0034803
Name: hsa_circ_TMEM87A
Synonym: circTMEM87A
Host Gene: TMEM87A
Genomic location(hg19): chr15:42553155-42560230:-
Genomic location(hg38): chr15:42260957-42268032:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissues; adjacent normal tissues; implanted tumor tissues; lung tissues
Cell lines:

HGC27; BGC823; MKN45; SGC7901; MGC803; GES-1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ULK1, QKI
Associated microRNA: miR-142-5p
Biological function: Promotes GC cell proliferation, migration, invasion, autophagy, tumor growth and metastasis, and attenuates apoptosis.
Molecular mechanism: circTMEM87A acts as a ceRNA by sponging miR-142-5p, reducing miR-142-5p-mediated repression of ULK1, thereby up-regulating ULK1 and promoting autophagy and GC progression; its high expression is at least partly caused by QKI up-regulation.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); EMT (promotes); autophagy (promotes); ceRNA regulation (promotes)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; IHC (Immunohistochemistry); Western Blot; In Vivo Animal Model; H&E Staining; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circTMEM87A expression is correlated with poor prognosis, worse 3-year OS, tumor size and lymph node metastasis in GC patients; circTMEM87A might be a prognostic biomarker and therapeutic target for GC.
Description:

circTMEM87A is up-regulated in gastric cancer tissues and cell lines, and high expression predicts poorer 3-year overall survival. Functionally, circTMEM87A promotes GC cell proliferation, migration, invasion, EMT, autophagy, xenograft tumor growth and metastasis while inhibiting apoptosis. Mechanistically, circTMEM87A acts as a miR-142-5p sponge to elevate ULK1 expression, and its formation is at least partly promoted by QKI.
Confidence score:

0.8778
Other information
Title:

Circular RNA TMEM87A promotes cell proliferation and metastasis of gastric cancer by elevating ULK1 via sponging miR-142-5p.

Journal: Journal of gastroenterology
Published: 2021
PubMed ID: 33155080
Study type:

combined biological and clinical study
Data availability: Electronic Supplementary Material (Online Resource 1); supplemental file 1: Table S1; Online Resource 2; Online Resource 3; Online Resource 4; Online Resource 5; Online Resource 6; Online Resource 7; Online Resource 8
Code availability: -