| Expression pattern: |
UP |
| Associated gene: |
GPX4 |
| Associated microRNA: |
miR-541-3p |
| Biological function: |
CircIL4R promotes HCC tumorigenesis, cell proliferation, and tumor growth, inhibits apoptosis, and suppresses erastin-induced ferroptosis by reducing iron accumulation and oxidative stress. |
| Molecular mechanism: |
CircIL4R functions as a ceRNA by sponging miR-541-3p, thereby positively regulating GPX4 expression and inhibiting ferroptosis in HCC. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ferroptosis (inhibits); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; Western Blot; Nuclear-Cytoplasmic Fractionation; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circIL4R expression was associated with worse overall survival in HCC patients, suggesting prognostic significance. |
| Description: |
CircIL4R is up-regulated in HCC tissues and cell lines and high expression is associated with poorer overall survival. Functionally, circIL4R promotes HCC proliferation and tumor growth while inhibiting apoptosis and erastin-induced ferroptosis. Mechanistically, it acts as a sponge for miR-541-3p to positively regulate GPX4 expression, forming the circIL4R/miR-541-3p/GPX4 axis. |
| Confidence score: |
0.8568 |