circRNA basic information
circBase ID: -
Name: hsa_circ_HIPK3
Synonym: CircHIPK3
Host Gene: HIPK3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005311
MONDO name: atherosclerosis
Disease details: atherosclerosis
Disease DO ID:
 1936
Disease MeSH ID:
 D050197
Disease NCIt ID:
 C35768
Disease ICD11 ID:
 109367356
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

arterial tissue; blood samples; femoral artery
Cell lines:

VSMCs
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: MFN2
Associated microRNA: miR-106a-5p
Biological function: Overexpression of circHIPK3 inhibits VSMC calcification, reduces mineralization and calcium content, and suppresses osteogenic and chondrogenic differentiation.
Molecular mechanism: circHIPK3 acts as a molecular sponge for miR-106a-5p and indirectly upregulates MFN2, thereby regulating VSMC calcification via the miR-106a-5p/MFN2 axis.
Biological pathway or process:

ceRNA regulation (promotes); other pathway/process (inhibits)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; Bioinformatics Analysis
Clinical significance:

CircHIPK3 may provide a new target gene and therapeutic strategy for atherosclerosis caused by vascular calcification.
Description:

circHIPK3 is downregulated in atherosclerotic tissues, blood samples, and calcification-associated VSMC differentiation models. Functionally, circHIPK3 overexpression suppresses VSMC mineralization, calcium deposition, osteogenic differentiation, and chondrogenic differentiation. Mechanistically, circHIPK3 sponges miR-106a-5p to relieve repression of MFN2, forming a circHIPK3/miR-106a-5p/MFN2 regulatory axis that inhibits vascular calcification.
Confidence score:

0.6675
Other information
Title:

CircHIPK3 Regulates Vascular Smooth Muscle Cell Calcification Via the miR-106a-5p/MFN2 Axis.

Journal: Journal of cardiovascular translational research
Published: 2022
PubMed ID: 35467292
Study type:

combined biological and clinical study
Data availability: All data generated or analyzed during this study are included in this article; datasets available from the corresponding author on reasonable request; supplementary material available at https:// doi. org/ 10. 1007/ s12265-022-10247-8
Code availability: -