circRNA basic information
circBase ID: -
Name: hsa_circ_SETD3
Synonym: circSETD3
Host Gene: SETD3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0015459
MONDO name: nasopharyngeal carcinoma
Disease details: nasopharyngeal carcinoma
Disease DO ID:
 9261
Disease MeSH ID:
 D00007727; D000077274
Disease NCIt ID:
 C3871
Disease ICD11 ID:
 1883313543
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NPC biopsy samples; NPC samples; non-cancerous nasopharyngeal epithelial (NPE) samples; paraffin-embedded specimens of NPC patients; lung tissues
Cell lines:

5-8F; NP69; CNE2; HNE2; HONE1; NPC cell lines
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: MAPRE1 mRNA, MAPRE1, alpha-tubulin
Associated microRNA: miR-615-5p, miR-1538
Biological function: circSETD3 promotes NPC cell invasion, migration, lung metastasis, dynamic microtubule assembly, and EMT; knockdown of circSETD3 inhibits cell migration, invasion, and lung metastasis.
Molecular mechanism: circSETD3 functions mainly in the cytoplasm as a ceRNA sponge for miR-615-5p and miR-1538, relieving their inhibition of MAPRE1 mRNA, upregulating MAPRE1, inhibiting alpha-tubulin acetylation, regulating dynamic cytoskeleton assembly, and activating EMT.
Biological pathway or process:

ceRNA regulation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); other pathway/process (promotes)
Detected method:
Q
H
S
Validation methods:

Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; ISH (In Situ Hybridization); IHC (Immunohistochemistry); IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Western Blot; Bioinformatics Analysis
Clinical significance:

circSETD3 is suggested as a novel molecular marker and potential target for NPC diagnosis and treatment.
Description:

circSETD3 is significantly upregulated in NPC cell lines and clinical tissues. It promotes NPC migration, invasion, and lung metastasis by acting as a cytoplasmic ceRNA sponge for miR-615-5p and miR-1538, thereby increasing MAPRE1 expression, reducing alpha-tubulin acetylation, promoting dynamic cytoskeleton assembly, and activating EMT. The study proposes circSETD3 as a potential diagnostic and therapeutic biomarker for NPC.
Confidence score:

0.8771
Other information
Title:

circSETD3 regulates MAPRE1 through miR-615-5p and miR-1538 sponges to promote migration and invasion in nasopharyngeal carcinoma.

Journal: Oncogene
Published: 2021
PubMed ID: 33122825
Study type:

combined biological and clinical study
Data availability: PRJNA391554; Supplementary Table S1; Supplementary Table S2; Supplementary Table S3; Supplementary Table S4; GSE12452
Code availability: -