circRNA basic information
circBase ID: hsa_circ_0064654
Name: hsa_circ_TGFBR2
Synonym: circTGFBR2(3-6)
Host Gene: TGFBR2
Genomic location(hg19): chr3:30686238-30715738:+
Genomic location(hg38): chr3:30644746-30674246:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005494
MONDO name: triple-negative breast carcinoma
Disease details: triple-negative breast carcinoma
Disease DO ID:
 0060081
Disease MeSH ID:
 D064726
Disease NCIt ID:
 C71732
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

MDA-MB-231; MCF10A-M2; MCF10A; A549; HEK293T; HACAT; MDA-MB-436; HCC38; BT549; SUM149PT; HCC1806; MDA-MB-231-BM
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: IGF2BP3, TGFBR1 mRNA, TGFBR1
Associated microRNA: -
Biological function: Enhances TGF-beta/SMAD signaling; promotes TGF-beta-induced EMT, cell migration, in vivo extravasation, stemness, and chemotherapeutic drug resistance in breast cancer cells; stabilizes TGFBR1 mRNA.
Molecular mechanism: circTGFBR2(3-6) acts as a non-coding scaffold that binds IGF2BP3 and TGFBR1 mRNA, strengthens IGF2BP3-TGFBR1 mRNA interaction, and stabilizes m6A-modified TGFBR1 mRNA to enhance TGF-beta/SMAD signaling.
Biological pathway or process:

TGF-beta/SMAD (promotes); EMT (promotes); migration (promotes); metastasis (promotes); stemness (promotes); chemoresistance (promotes); mRNA stability (promotes); m6A modification (other); other pathway/process (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Actinomycin D / DRB Stability Assay; RNA-seq; ISH (In Situ Hybridization); IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; MeRIP / MeRIP-seq; Transfection; Transwell Assay; In Vivo Animal Model; Western Blot; Bioinformatics Analysis
Clinical significance:

-
Description:

circTGFBR2(3-6), a TGFBR2-derived human circRNA, enhances breast cancer cell plasticity by potentiating TGF-beta/SMAD signaling. It acts as a non-coding scaffold for IGF2BP3 and TGFBR1 mRNA, promoting m6A-dependent TGFBR1 mRNA stabilization and thereby driving EMT, migration, extravasation, stemness, and chemotherapeutic drug resistance.
Confidence score:

0.8187
Other information
Title:

circTGFBR2(3-6) acts as an assembly platform for RNA-binding protein IGF2BP3 and TGFBR1 mRNA to enhance breast cancer cell plasticity.

Journal: Cell death and differentiation
Published: 2025
PubMed ID: 41145815
Study type:

biological research
Data availability: PXD061218; GSE303132; Supplementary Materials; https://doi.org/10.1038/s41418-025-01597-2
Code availability: -