| Expression pattern: |
DN |
| Associated gene: |
PDCD4 |
| Associated microRNA: |
miR-503-5p |
| Biological function: |
Suppresses CRC cell proliferation, migration and invasion; promotes apoptosis. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-503-5p to upregulate PDCD4. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Clinical Sample Validation |
| Clinical significance: |
circ_0003266 expression level was negatively correlated with tumor grade and stage of CRC patients; may be a biomarker and therapy target for CRC. |
| Description: |
circ_0003266 is down-regulated in colorectal cancer tissues and cell lines and functions as a tumor suppressor. It sponges miR-503-5p to relieve repression of PDCD4, thereby inhibiting proliferation/migration/invasion and promoting apoptosis in CRC cells. |
| Confidence score: |
0.7404 |