| Expression pattern: |
UP |
| Associated gene: |
FUS, SP1, beta-catenin, SOX2 |
| Associated microRNA: |
miR-346 |
| Biological function: |
promotes CRC cell proliferation, migration, EMT, and stemness |
| Molecular mechanism: |
Acts as a ceRNA sponge for miR-346 and recruits the RBP FUS to elevate/stabilize SP1; strengthens SP1-beta-catenin interaction to activate Wnt/beta-catenin signaling; feedback where SOX2 transcriptionally activates SP1 to boost circ_0026628. |
| Biological pathway or process: |
Wnt/beta-catenin (promotes); proliferation (promotes); migration (promotes); EMT (promotes); stemness (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; Agarose gel electrophoresis; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; IF (Immunofluorescence); RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; ChIP / ChIP-seq; Luciferase Reporter Assay |
| Clinical significance: |
- |
| Description: |
In colorectal cancer cells, circ_0026628 is upregulated and promotes proliferation, migration, EMT and stemness. It increases SP1 by sponging miR-346 and recruiting FUS to stabilize SP1 mRNA, thereby enhancing SP1/beta-catenin interaction and activating Wnt/beta-catenin signaling; SOX2 provides positive feedback by transcriptionally activating SP1 to elevate circ_0026628. |
| Confidence score: |
0.8155 |