circRNA basic information
circBase ID: hsa_circ_0000517
Name: hsa_circ_RPPH1
Synonym: hsa_circRNA_001678
Host Gene: RPPH1
Genomic location(hg19): chr14:20811404-20811492:-
Genomic location(hg38): chr14:20343245-20343333:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

breast cancer tissues; normal breast tissues
Cell lines:

SKBR3; BT-549; MCF-7; MDA-MB-231; MCF-10A
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: Ago2, BBC3, ZNF212, RBM10, SIRT6, CCDC124, CIAO3, EPN1, DUSP23, C7orf50, RPL28, PWWP2B, NCLN, KLF16, ZNHIT1, RFXANK, DDX54, GIPC1
Associated microRNA: miR-326
Biological function: hsa_circ_0000517 promotes breast cancer cell proliferation, colony formation and invasion, and inhibits apoptosis; hsa_circ_0000517 knockdown suppresses these malignant behaviors and triggers apoptosis.
Molecular mechanism: hsa_circ_0000517 functions as a cytoplasmic molecular sponge of miR-326, suppressing miR-326 expression; miR-326 inhibition reverses the effects of hsa_circ_0000517 knockdown, and 17 genes were predicted as potential miR-326 targets in breast cancer.
Biological pathway or process:

proliferation (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (other)
Detected method:
Q
M
Validation methods:

RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis
Clinical significance:

The hsa_circ_0000517/miR-326 regulatory axis may provide valuable insights into breast cancer carcinogenesis and progression.
Description:

hsa_circ_0000517 is upregulated in breast cancer tissues and cells and behaves as an oncogenic circRNA. It is stable, cytoplasm-enriched, and promotes breast cancer cell proliferation, colony formation and invasion while suppressing apoptosis, mainly by sponging miR-326 and reducing miR-326 expression. The study also predicted 17 potential miR-326 downstream targets, suggesting a hsa_circ_0000517/miR-326/mRNA regulatory axis in breast cancer progression.
Confidence score:

0.7216
Other information
Title:

The effects of hsa_circ_0000517/miR-326 axis on the progression of breast cancer cells and the prediction of miR-326 downstream targets in breast cancer.

Journal: Pathology, research and practice
Published: 2021
PubMed ID: 34619576
Study type:

combined biological and bioinformatics study
Data availability: GSE101123; TCGA; GTEx; LinkedOmics; Starbase; circular RNA interactome; Supplementary data associated with this article can be found in the online version at doi:10.1016/j.prp.2021.153638
Code availability: -