circRNA basic information
circBase ID: hsa_circ_0065214
Name: hsa_circ_SCAP
Synonym: circSCAP
Host Gene: SCAP
Genomic location(hg19): chr3:47466974-47476627:-
Genomic location(hg38): chr3:47425484-47435137:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

lung tumor tissues; peritumor tissues / adjacent normal tissues

Cell lines:

H1650; A549; H1299; PC9; HCC827; 16HBE; 293-T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
DN
Associated gene: SF3A3, PRMT5, MDM4-S, p53
Associated microRNA: miR-365b-3p
Biological function: inhibits proliferation; inhibits migration; promotes apoptosis; suppresses tumor growth/malignance
Molecular mechanism: circSCAP binds SF3A3 and promotes its ubiquitin-proteasome-mediated degradation, increases MDM4-S alternative splicing and activates downstream p53 signaling; also sponges miR-365b-3p but not functionally through it
Biological pathway or process:

p53 signaling (promotes); proliferation (inhibits); migration (inhibits); apoptosis (promotes); cell cycle (inhibits); ubiquitination (promotes)

Detected method:
Q
B
M
Validation methods:

Microarray; RT-qPCR; Northern Blot; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Clinical Sample Validation; Survival Analysis; ROC Analysis; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Western Blot; Co-IP; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry)

Clinical significance:

Low circSCAP level is associated with poor prognosis; circSCAP is an independent prognostic factor and a robust biomarker for prognosis evaluation in lung cancer

Description:

circSCAP (hsa_circ_0065214), derived from SCAP, is down-regulated in lung cancer/NSCLC and acts as a tumor suppressor. It binds SF3A3 and promotes SF3A3 ubiquitin-proteasome degradation, increasing MDM4-S and activating p53 signaling to inhibit proliferation/migration and promote apoptosis. Higher circSCAP expression is associated with better patient prognosis and has prognostic biomarker potential.

Confidence score:

0.8913

Other information
Title:

CircSCAP interacts with SF3A3 to inhibit the malignance of non-small cell lung cancer by activating p53 signaling.

Journal: Journal of experimental & clinical cancer research : CR
Published: 2022
PubMed ID: 35365208
Study type:

combined biological and clinical study

Data availability: available upon request
Code availability: -