| Expression pattern: |
DN |
| Associated gene: |
TWIST1 |
| Associated microRNA: |
miR-25-3p |
| Biological function: |
TGFBR2 suppresses osteoblast differentiation of human aortic valve interstitial cells, decreasing ALP activity, calcium nodule formation, and RUNX2 and OPN expression. |
| Molecular mechanism: |
TGFBR2 acts as a ceRNA/miRNA sponge for miR-25-3p to regulate TWIST1 expression, thereby inhibiting osteoblast differentiation in CAVD-related human aortic VICs. |
| Biological pathway or process: |
ceRNA regulation (other); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; Western Blot |
| Clinical significance: |
TGFBR2 could become a novel theoretical basis for the treatment of CAVD. |
| Description: |
In CAVD, circRNA TGFBR2 is down-regulated in calcific aortic valves and osteogenically induced human VICs. The study shows that TGFBR2 functions as a miR-25-3p sponge to regulate TWIST1, thereby suppressing osteoblast differentiation, ALP activity, calcified nodule formation, and RUNX2/OPN expression. |
| Confidence score: |
0.6102 |