circRNA basic information
circBase ID: -
Name: hsa_circ_CPA4
Synonym: Circular RNA carboxypeptidase A4
Host Gene: CPA4
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0021042
MONDO name: glioma
Disease details: glioma
Disease DO ID:
 -
Disease MeSH ID:
 D005910
Disease NCIt ID:
 C3059
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

glioma tissues; normal brain tissues; tumor masses
Cell lines:

A-172; LN-229; NHA
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: MEF2D, MMP2, MMP9
Associated microRNA: miR-760
Biological function: CircCPA4 promotes glioma cell proliferation, migration, invasion, tumor growth, and radioresistance, while inhibiting apoptosis; circCPA4 knockdown suppresses malignant phenotypes and enhances apoptosis and radiosensitivity.
Molecular mechanism: CircCPA4 functions as a ceRNA by sponging miR-760, thereby indirectly up-regulating MEF2D expression and promoting malignant phenotypes in glioma.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); radioresistance (promotes)
Detected method:
Q
Validation methods:

Actinomycin D / DRB Stability Assay; RNase R Treatment; RT-qPCR; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Higher circCPA4 expression was correlated with WHO grade and tumor size, and high circCPA4 expression predicted shorter overall survival and unfavorable prognosis in glioma patients.
Description:

CircCPA4 is up-regulated in glioma tissues and cell lines and high expression is associated with higher WHO grade, larger tumor size, and shorter overall survival. Functionally, circCPA4 promotes glioma proliferation, migration, invasion, tumor growth, and radioresistance while suppressing apoptosis. Mechanistically, it acts as a ceRNA for miR-760 to regulate MEF2D, forming a circCPA4/miR-760/MEF2D axis that drives glioma progression.
Confidence score:

0.8298
Other information
Title:

CircCPA4 Promotes the Malignant Phenotypes in Glioma via miR-760/MEF2D Axis.

Journal: Neurochemical research
Published: 2020
PubMed ID: 33068224
Study type:

combined biological and clinical study
Data availability: -
Code availability: -