| Expression pattern: |
UN |
| Associated gene: |
SMAD5 |
| Associated microRNA: |
miR-4781-3p |
| Biological function: |
promotes osteogenic/osteoblastic differentiation of PDLSCs and periodontal bone regeneration; does not significantly affect PDLSC proliferation |
| Molecular mechanism: |
ceRNA/miRNA sponge mechanism: circFAT1 sponges miR-4781-3p to regulate SMAD5 |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (other); osteogenesis (other pathway/process) (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; Transfection; Western Blot; EdU; CCK8; In Vivo Animal Model; H&E Staining |
| Clinical significance: |
potential target for PDLSCs-mediated periodontal bone regeneration |
| Description: |
circFAT1 promotes osteogenic/osteoblastic differentiation of human PDLSCs and supports bone regeneration, mainly by acting as a miRNA sponge for miR-4781-3p to relieve repression of SMAD5. circFAT1 is predominantly localized in the cytoplasm and does not significantly affect PDLSC proliferation. |
| Confidence score: |
0.589 |