| Expression pattern: |
UP |
| Associated gene: |
TRIM31 |
| Associated microRNA: |
miR-876-3p |
| Biological function: |
circNFIX promotes AML cell proliferation and cell cycle progression and inhibits apoptosis; circNFIX knockdown suppresses proliferation, induces G0/G1 cell cycle arrest, and promotes apoptosis in AML cells. |
| Molecular mechanism: |
circNFIX acts as a miR-876-3p sponge and indirectly up-regulates TRIM31 expression through miR-876-3p, thereby promoting AML cell malignant progression. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; ROC Analysis; Transfection; MTT; EdU Staining; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; Bioinformatics Analysis |
| Clinical significance: |
circNFIX may have diagnostic value for AML, with serum circNFIX ROC AUC of 0.927, sensitivity of 83%, and specificity of 97.5%; circNFIX may be an effective target for future AML therapy. |
| Description: |
circNFIX is up-regulated in AML bone marrow samples and AML cell lines and may serve as a diagnostic biomarker. Functionally, circNFIX promotes AML cell proliferation and cell cycle progression while suppressing apoptosis. Mechanistically, circNFIX sponges miR-876-3p to up-regulate TRIM31 expression, forming a circNFIX/miR-876-3p/TRIM31 axis that supports AML progression. |
| Confidence score: |
0.7548 |