| Expression pattern: |
UP |
| Associated gene: |
HRAS, Ago2 |
| Associated microRNA: |
miR-338-3p |
| Biological function: |
Promotes NPC cell proliferation, migration, invasion, glycolysis, and tumor growth in vivo; knockdown suppresses these malignant phenotypes. |
| Molecular mechanism: |
circZNF609 acts as a competing endogenous RNA for miR-338-3p, thereby regulating HRAS expression and promoting oncogenic phenotypes and glycolysis in NPC. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); glycolysis (promotes); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis; ELISA |
| Clinical significance: |
High circZNF609 expression had a poor prognosis in NPC patients and circZNF609 may be a therapeutic target for targeted molecular therapies of NPC. |
| Description: |
circZNF609 is up-regulated in NPC tissues and cell lines, and high expression is associated with poor overall survival. Functionally, circZNF609 promotes NPC cell proliferation, migration, invasion, glycolysis, and xenograft tumor growth. Mechanistically, it acts as a ceRNA that sponges miR-338-3p to increase HRAS expression. |
| Confidence score: |
0.8174 |