circRNA basic information
circBase ID: hsa_circ_0000463
Name: hsa_circ_EP400NL
Synonym: -
Host Gene: EP400NL
Genomic location(hg19): chr12:132609079-132609271:-
Genomic location(hg38): chr12:132124534-132124726:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non‐small cell lung cancer
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; adjacent healthy tissues; xenograft tumors
Cell lines:

NCI-H1299; HCC827; A549; H460; 16HBE
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SLC1A5, Ago2
Associated microRNA: miR-924
Biological function: promotes proliferation, migration, invasion, EMT and glutamine metabolism; inhibits apoptosis; promotes xenograft tumor growth
Molecular mechanism: ceRNA sponge mechanism: circ_0000463 sponges miR-924 to upregulate SLC1A5, thereby promoting malignant behaviors and glutamine metabolism in NSCLC
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); EMT (promotes); glutaminolysis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Transfection; Colony Formation Assay; EdU Staining; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Western Blot; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis
Clinical significance:

-
Description:

circ_0000463 is up-regulated in NSCLC tissues and cell lines and promotes malignant phenotypes (proliferation, migration, invasion/EMT) while inhibiting apoptosis. Mechanistically, it functions as a ceRNA by sponging miR-924 to increase SLC1A5 expression, thereby enhancing glutamine metabolism and supporting tumor growth in a cell line-derived xenograft model.
Confidence score:

0.8299
Other information
Title:

Circ_0000463 contributes to the progression and glutamine metabolism of non-small-cell lung cancer by targeting miR-924/SLC1A5 signaling.

Journal: Journal of clinical laboratory analysis
Published: 2022
PubMed ID: 34811815
Study type:

combined biological and clinical study
Data availability: The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -