circRNA basic information
circBase ID: hsa_circ_0001649
Name: hsa_circ_SHPRH
Synonym: circ_0001649
Host Gene: -
Genomic location(hg19): chr6:146209155-146216113:-
Genomic location(hg38): chr6:145888019-145894977:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: Osteosarcoma
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

OS tissues; paired adjacent non-tumor tissues
Cell lines:

MG63; U2OS; HOS; Saos-2; hFOB1.19; 293 T
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: STAT3, STAT5, CDK4, CDK6, Apaf-1, cleaved-caspase-3, cleaved-caspase-9
Associated microRNA: miR-338-5p, miR-647, miR-942
Biological function: circ_0001649 suppresses OS cell viability, survival fraction and proliferation, promotes apoptosis, and inhibits STAT signal pathway activation.
Molecular mechanism: circ_0001649 functions as a miRNA sponge for miR-338-5p, miR-647 and miR-942, thereby suppressing OS cell proliferation, inducing apoptosis and inhibiting the STAT signal pathway.
Biological pathway or process:

JAK/STAT (inhibits); apoptosis (promotes); proliferation (inhibits); cell cycle (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot
Clinical significance:

circ_0001649 might provide a new latent therapeutic goal for monitoring and treating OS.
Description:

In this study, hsa_circ_0001649/circ_0001649 was down-regulated in osteosarcoma tissues and cell lines. Functional experiments showed that circ_0001649 overexpression suppressed OS cell viability, colony formation and proliferation, promoted apoptosis, and inhibited STAT signaling. Mechanistically, circ_0001649 acted as a sponge for miR-338-5p, miR-647 and miR-942, suggesting a tumor-suppressive role and potential therapeutic relevance in OS.
Confidence score:

0.6521
Other information
Title:

Circular RNA hsa_circ_0001649 suppresses the growth of osteosarcoma cells via sponging multiple miRNAs.

Journal: Cell cycle (Georgetown, Tex.)
Published: 2020
PubMed ID: 32954926
Study type:

combined biological and clinical study
Data availability: available from the corresponding author on reasonable request
Code availability: -