| Expression pattern: |
UP |
| Associated gene: |
EZR mRNA, EZR, TPM3, RhoA |
| Associated microRNA: |
- |
| Biological function: |
Promotes NPC cell migration, invasion, lung metastasis, filopodia extension, microfilament accumulation, EMT marker changes, and cytoskeletal remodeling. |
| Molecular mechanism: |
circARHGAP12 directly binds the EZR mRNA 3'UTR and promotes EZR mRNA stability; EZR forms a complex with TPM3 and RhoA, creating a feedback regulatory loop that drives microfilament aggregation and cytoskeletal remodeling. |
| Biological pathway or process: |
migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); mRNA stability (promotes); other pathway/process (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; Actinomycin D / DRB Stability Assay; RT-qPCR; ISH (In Situ Hybridization); IHC (Immunohistochemistry); IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Luciferase Reporter Assay; Transfection; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circARHGAP12 may serve as a potential biomarker for NPC treatment, but its prognostic value remains unclear. |
| Description: |
circARHGAP12 is up-regulated in nasopharyngeal carcinoma tissues and cell lines. It promotes NPC migration, invasion, lung metastasis, EMT-related changes, and cytoskeletal remodeling by directly binding the EZR mRNA 3'UTR and enhancing EZR mRNA stability, thereby activating an EZR/TPM3/RhoA feedback regulatory complex. The study suggests circARHGAP12 may be a potential biomarker and therapeutic target in NPC. |
| Confidence score: |
0.8855 |