circRNA basic information
circBase ID: hsa_circ_0033546
Name: hsa_circ_AKT1
Synonym: circAKT1
Host Gene: AKT1
Genomic location(hg19): chr14:105235686-105239429:-
Genomic location(hg38): chr14:104769349-104773092:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005005
MONDO name: clear cell renal carcinoma
Disease details: clear cell renal cell carcinoma
Disease DO ID:
 4467
Disease MeSH ID:
 -
Disease NCIt ID:
 C4033
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

ccRCC tumor tissues; adjacent normal tissues; adjacent noncancerous tissues
Cell lines:

HK-2; 786-O; A498; ACHN; Caki-1; OS-RC-2
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: CAV1, AKT1 mRNA
Associated microRNA: miR-338e3p, miR-942e5p
Biological function: circAKT1 promotes ccRCC cell proliferation, colony formation, migration, invasion and EMT; circAKT1 knockdown suppresses these malignant phenotypes in vitro.
Molecular mechanism: circAKT1 acts as a cytoplasmic ceRNA/miRNA sponge for miR-338e3p, preventing miR-338e3p-mediated suppression of CAV1 and thereby up-regulating CAV1 expression.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circAKT1 expression was positively associated with TNM stage, lymph node metastasis and predicted an adverse prognosis; circAKT1 was proposed as a potential prognostic biomarker and therapeutic target in ccRCC.
Description:

circAKT1 is up-regulated in ccRCC tissues and cell lines, and high expression is associated with advanced TNM stage, lymph node metastasis, and poor overall survival. Functionally, circAKT1 promotes proliferation, colony formation, migration, invasion, and EMT in ccRCC cells. Mechanistically, circAKT1 acts as a cytoplasmic sponge for miR-338e3p to release CAV1 from miRNA-mediated repression, thereby facilitating ccRCC progression.
Confidence score:

0.8389
Other information
Title:

CircAKT1 acts as a sponge of miR-338-3p to facilitate clear cell renal cell carcinoma progression by up-regulating CAV1.

Journal: Biochemical and biophysical research communications
Published: 2020
PubMed ID: 32900491
Study type:

combined biological and clinical study
Data availability: GSE100186; https://doi.org/10.1016/j.bbrc.2020.08.081
Code availability: -