| Expression pattern: |
UP |
| Associated gene: |
SOX4 |
| Associated microRNA: |
miRNA-338-3p |
| Biological function: |
Promotes NSCLC cell proliferation, invasion/metastasis, and drug resistance; circ_0005909 knockdown suppresses these behaviors and reduces xenograft tumor growth. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miRNA-338-3p, thereby relieving repression of SOX4. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); metastasis (promotes); drug resistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; EdU Staining; Transwell Assay; In Vivo Animal Model; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0005909 expression is associated with poor prognosis and shorter overall survival in NSCLC patients; independently associated with overall survival. |
| Description: |
hsa_circ_0005909 (circ_0005909), derived from XPR1, is up-regulated in NSCLC tissues and cell lines and its high expression predicts shorter overall survival. Functionally it promotes proliferation, invasion/metastasis and adriamycin-related drug resistance, and its knockdown inhibits xenograft tumor growth. Mechanistically, it acts as a ceRNA by sponging miRNA-338-3p to increase SOX4 expression. |
| Confidence score: |
0.8353 |