| Expression pattern: |
DN |
| Associated gene: |
PTEN, PI3K, Akt |
| Associated microRNA: |
miR-148b-3p |
| Biological function: |
circ_0000442 functions as a tumor suppressor in breast cancer, reducing cell viability and colony formation, inducing G1 arrest, and suppressing tumor growth. |
| Molecular mechanism: |
circ_0000442 acts as a sponge of miR-148b-3p, blocks miR-148b-3p targeting of PTEN, increases PTEN, and inhibits PI3K/Akt signaling. |
| Biological pathway or process: |
PI3K/AKT (inhibits); proliferation (inhibits); cell cycle (other); ceRNA regulation (promotes); other pathway/process (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNA-seq; Bioinformatics Analysis; Transfection; Colony Formation Assay; Cell Cycle Assay; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; In Vivo Animal Model |
| Clinical significance: |
Circ_0000442 hold the promise of being developed as a novel biomarker of breast cancer. |
| Description: |
hsa_circ_0000442/circ_0000442 is down-regulated in breast cancer tissues and cell lines. It acts as a tumor suppressor by sponging miR-148b-3p, thereby relieving miR-148b-3p-mediated repression of PTEN and inhibiting PI3K/Akt signaling, which reduces proliferation, colony formation, and xenograft tumor growth. |
| Confidence score: |
0.657 |