| Expression pattern: |
DN |
| Associated gene: |
ATF3 |
| Associated microRNA: |
miR-145-5p |
| Biological function: |
attenuates cardiac hypertrophy; protects cardiomyocytes against Ang II-induced hypertrophy; alleviates TAC-induced cardiac dysfunction; inhibits cardiomyocyte apoptosis |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-145-5p, thereby relieving miR-145-5p-mediated repression of ATF3 (ceRNA mechanism). |
| Biological pathway or process: |
proliferation (not specified); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; Transfection; IF (Immunofluorescence); Western Blot; In Vivo Animal Model; H&E Staining; TUNEL; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
circNfix and ATF3 were positively correlated in patients with cardiac hypertrophy; circNfix may serve as a molecular target for cardiac hypertrophy treatment |
| Description: |
circNfix (circBase: hsa_circ_0005660, from NFIX exon 2) is down-regulated in Ang II-treated cardiomyocytes and in TAC-induced hypertrophic mouse hearts. Its overexpression attenuates cardiac hypertrophy and cardiomyocyte apoptosis mainly by sponging miR-145-5p to de-repress ATF3 (circNfix/miR-145-5p/ATF3 axis). |
| Confidence score: |
0.8351 |