| Expression pattern: |
UP |
| Associated gene: |
cyclin D1, PCNA, TGF-beta1, Col. I, FN |
| Associated microRNA: |
miR-185 |
| Biological function: |
circHIPK3 promotes diabetic nephropathy progression, mesangial cell proliferation, renal fibrosis-associated gene expression, and renal function damage under diabetic/high-glucose conditions. |
| Molecular mechanism: |
circHIPK3 acts as a miRNA sponge for miR-185; circHIPK3 silencing increases miR-185 and reduces cyclin D1, PCNA, TGF-beta1, Col. I, and FN expression, thereby inhibiting proliferation and ameliorating DN progression. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (promotes); cell cycle (promotes); fibrosis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Luciferase Reporter Assay; Transfection; CCK8; Western Blot; In Vivo Animal Model; ELISA |
| Clinical significance: |
circHIPK3 might be a biomarker or therapeutic target for DN; circHIPK3 may be used as a biomarker for early diagnosis of DN and a potential target for DN treatment. |
| Description: |
circHIPK3 is up-regulated in renal tissues of diabetic nephropathy mice and in high-glucose-treated rat mesangial cells. Functionally, circHIPK3 promotes mesangial cell proliferation and DN progression by sponging miR-185 and regulating fibrosis- and proliferation-associated factors including TGF-beta1, Col. I, FN, cyclin D1, and PCNA. The study proposes circHIPK3 as a potential early diagnostic biomarker and therapeutic target for DN. |
| Confidence score: |
0.528 |