circRNA basic information
circBase ID: hsa_circ_0000034
Name: hsa_circ_DHDDS
Synonym: circDHDDS
Host Gene: DHDDS
Genomic location(hg19): chr1:26772806-26774151:+
Genomic location(hg38): chr1:26446315-26447660:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008380
MONDO name: retinoblastoma
Disease details: Retinoblastoma
Disease DO ID:
 768
Disease MeSH ID:
 D012175
Disease NCIt ID:
 C7541
Disease ICD11 ID:
 1855353671
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

RB tissues; normal retinas tissues; tumor tissues
Cell lines:

RPCs; Y-79; WERI-Rb-1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: WNT3A
Associated microRNA: miR-361-3p
Biological function: Promotes RB cell proliferation, migration, invasion, cell cycle progression, stemness-associated marker expression, and tumor growth in vivo.
Molecular mechanism: circDHDDS acts as a miR-361-3p sponge to regulate WNT3A expression, thereby promoting RB development.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); stemness (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

Potential therapeutic or functional target perspective for RB; diagnostic value was suggested as requiring future verification.
Description:

circDHDDS is up-regulated in retinoblastoma tissues and cells. It promotes RB cell proliferation, migration, invasion, cell cycle progression, stemness-related marker expression, and xenograft tumor growth by sponging miR-361-3p and increasing WNT3A expression.
Confidence score:

0.7416
Other information
Title:

CircDHDDS/miR-361-3p/WNT3A Axis Promotes the Development of Retinoblastoma by Regulating Proliferation, Cell Cycle, Migration, and Invasion of Retinoblastoma Cells.

Journal: Neurochemical research
Published: 2020
PubMed ID: 32865704
Study type:

combined biological and clinical study
Data availability: available from the corresponding author on reasonable request
Code availability: -