| Expression pattern: |
UP |
| Associated gene: |
- |
| Associated microRNA: |
miR-1253, miR-1324, miR-409-3p, miR-578 |
| Biological function: |
Promotes ESCC cell proliferation, migration and invasion; inhibits apoptosis; affects cell cycle progression (G2 arrest upon knockdown). |
| Molecular mechanism: |
Downstream miRNAs were predicted bioinformatically as potential binding partners, but no experimental mechanism was validated. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Clinical Sample Validation; ROC Analysis; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Bioinformatics Analysis |
| Clinical significance: |
High expression of circ_0014715 was related to nerve invasion, vascular invasion, more advanced tumor-node-metastasis (TNM) stage and poor differentiation grade; ROC curves suggested diagnostic value for ESCC. |
| Description: |
circ_0014715 (from host gene CCT3) is up-regulated in ESCC tissues and cell lines. Functional assays show it promotes proliferation, migration and invasion and inhibits apoptosis, with knockdown causing G2-phase arrest. The study only provides bioinformatic predictions of candidate binding miRNAs without mechanistic validation, and suggests potential diagnostic value by ROC analysis. |
| Confidence score: |
0.7554 |