| Expression pattern: |
UP |
| Associated gene: |
AKAP2 |
| Associated microRNA: |
miR-516b-5p |
| Biological function: |
Circ-SLC8A1 promotes osteoporosis progression in BMSCs, enhances mineralized nodule formation and calcium deposits, and activates osteoporosis-related osteogenic markers including ALP, BMP4, SPP1 and BGLAP. |
| Molecular mechanism: |
Circ-SLC8A1 functions as a ceRNA that sponges miR-516b-5p, thereby relieving the inhibitory effect of miR-516b-5p on AKAP2 expression and modulating osteoporosis progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circ-SLC8A1 may function as a novel biomarker in treating osteoporosis. |
| Description: |
Circ-SLC8A1 is up-regulated in the OVX osteoporosis model and OVX-derived BMSCs. It promotes osteoporosis-related cellular phenotypes by acting as a cytoplasmic ceRNA that binds miR-516b-5p and increases AKAP2 expression, forming the circ-SLC8A1/miR-516b-5p/AKAP2 regulatory axis. |
| Confidence score: |
0.7549 |