| Expression pattern: |
UP |
| Associated gene: |
TRIM24 / tripartite motif-containing protein 24 |
| Associated microRNA: |
miR-1248 |
| Biological function: |
circEPSTI1 promotes NSCLC cell proliferation, colony formation and invasion; knockdown of circEPSTI1 represses proliferation and invasion and exerts an antitumor effect. |
| Molecular mechanism: |
circEPSTI1 acts as a sponge of miR-1248, decreasing miR-1248 activity and thereby relieving miR-1248-mediated suppression of TRIM24; downregulation of circEPSTI1 inhibits TRIM24 via miR-1248 upregulation. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circEPSTI1 is up-regulated in NSCLC tissues and cell lines and functions as an oncogenic circRNA. Its knockdown suppresses NSCLC cell proliferation, colony formation and invasion by increasing miR-1248 and reducing the miR-1248 target TRIM24, defining a circEPSTI1-miR-1248-TRIM24 ceRNA axis. |
| Confidence score: |
0.7032 |