| Expression pattern: |
UP |
| Associated gene: |
LASP1 / LIM and SH3 protein 1 |
| Associated microRNA: |
miR-330-5p |
| Biological function: |
circ-FARSA promotes CRC cell proliferation, migration, invasion and in vivo tumor growth; knockdown inhibits these malignant phenotypes. |
| Molecular mechanism: |
circ-FARSA functions as a ceRNA/miRNA sponge for miR-330-5p, thereby regulating LASP1 expression through the miR-330-5p/LASP1 axis. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Western Blot; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circ-FARSA expression had a significant association with overall survival of CRC patients; higher expression was associated with shorter overall survival, and circ-FARSA might be explored as a diagnostic marker or therapeutic target. |
| Description: |
circ-FARSA is up-regulated in colorectal cancer tissues and cell lines, and high expression is associated with poorer overall survival. Functionally, circ-FARSA promotes CRC cell proliferation, migration, invasion and xenograft tumor growth. Mechanistically, it acts as a sponge for miR-330-5p to regulate LASP1 expression through the circ-FARSA/miR-330-5p/LASP1 axis. |
| Confidence score: |
0.8005 |