| Expression pattern: |
UP |
| Associated gene: |
YY1, Ki-67, Ago2, Cyclin A1, PCNA, Bax, Cleaved Caspase-3 |
| Associated microRNA: |
miR-215-5p |
| Biological function: |
Promotes melanoma cell proliferation and cell viability; suppresses melanoma cell apoptosis; promotes melanoma progression. |
| Molecular mechanism: |
circHIPK3 acts as a ceRNA sponge for miR-215-5p to upregulate YY1, thereby promoting proliferation and inhibiting apoptosis in melanoma cells. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); cell cycle (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Bioinformatics Analysis; Transfection; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot |
| Clinical significance: |
circHIPK3 expression was positively correlated with Ki-67 and may serve as a novel therapeutic target or potential diagnostic and prognostic index for melanoma. |
| Description: |
circHIPK3 is upregulated in melanoma tissues and cell lines and appears to act as an oncogenic circRNA. Mechanistically, it sponges miR-215-5p and increases YY1 expression, thereby promoting melanoma cell proliferation and suppressing apoptosis. Its expression correlates positively with Ki-67, suggesting potential relevance to melanoma progression and therapeutic targeting. |
| Confidence score: |
0.7945 |