| Expression pattern: |
UP |
| Associated gene: |
YAP1 |
| Associated microRNA: |
miR-30a-5p |
| Biological function: |
Exosomal circ-PVT1 promotes cisplatin resistance in gastric cancer cells by inducing autophagy and invasion and inhibiting apoptosis; circ-PVT1 knockdown enhances DDP sensitivity and suppresses tumor growth in vivo. |
| Molecular mechanism: |
Exosomal circ-PVT1 acts as a miR-30a-5p sponge and indirectly upregulates YAP1, thereby regulating autophagy, invasion, apoptosis, and DDP resistance. |
| Biological pathway or process: |
autophagy (promotes); invasion (promotes); apoptosis (inhibits); chemoresistance (promotes); drug resistance (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; MTT; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
High serum exosomal circ-PVT1 was positively correlated with TNM grade, lymph node metastasis, and tumor size; exosomal circ-PVT1 might be a prospective indicator and promising diagnostic biomarker for DDP therapy/GC treatment. |
| Description: |
This study found that exosomal circ-PVT1 is upregulated in cisplatin-resistant gastric cancer serum exosomes and cells. Functionally, circ-PVT1 promotes cisplatin resistance by suppressing miR-30a-5p and indirectly increasing YAP1, thereby enhancing autophagy and invasion while inhibiting apoptosis; circ-PVT1 knockdown improves DDP sensitivity in vitro and in a cell line-derived xenograft model. |
| Confidence score: |
0.6536 |