| Expression pattern: |
UP |
| Associated gene: |
Notch-1, Hes1, DLL4, Notch signaling pathway |
| Associated microRNA: |
miR-375 |
| Biological function: |
hsa_circ_0008035 promotes osteosarcoma cell proliferation and migration and inhibits apoptosis; silencing hsa_circ_0008035 suppresses OSA cell growth and migration and promotes apoptosis. |
| Molecular mechanism: |
hsa_circ_0008035 functions as a sponge of miR-375 and negatively regulates miR-375 expression; silencing hsa_circ_0008035 perturbs the Notch signaling pathway by upregulating miR-375. |
| Biological pathway or process: |
Notch (promotes); proliferation (promotes); migration (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Wound Healing Assay; Western Blot |
| Clinical significance: |
hsa_circ_0008035 was expected to be a potential molecular biomarker for early diagnosis of OSA. |
| Description: |
hsa_circ_0008035 is up-regulated in serum, tissues and cell lines from osteosarcoma. Functionally, hsa_circ_0008035 promotes OSA cell growth and migration and suppresses apoptosis, while its silencing has antitumor effects. Mechanistically, it acts through miR-375 and the Notch signaling pathway, suggesting potential value as an early diagnostic biomarker or therapeutic target. |
| Confidence score: |
0.6521 |