| Expression pattern: |
UP |
| Associated gene: |
YY1, CAMLG, EZH2, SH3GL2, ZO-1, Occludin |
| Associated microRNA: |
miR-514a-5p |
| Biological function: |
promotes CCA cell proliferation, migration, invasion, tumorigenesis and metastasis; inhibits apoptosis; induces angiogenesis; disrupts vascular endothelial barriers and increases endothelial permeability |
| Molecular mechanism: |
circ-CCAC1 acts through a ceRNA-like circ-CCAC1/miR-514a-5p/YY1/CAMLG axis in CCA cells and, as an exosomal circRNA in endothelial cells, sequesters EZH2 in the cytoplasm to increase SH3GL2 and suppress ZO-1/Occludin signaling. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); angiogenesis (promotes); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
H
S
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; Microarray; FISH / smFISH; ISH (In Situ Hybridization); IHC (Immunohistochemistry); IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Tube Formation Assay; In Vivo Animal Model; H&E Staining; Western Blot; Cohort Study; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circ-CCAC1 in bile and serum EVs has diagnostic value; elevated tissue circ-CCAC1 correlates with adverse clinicopathological characteristics and is an independent prognostic and recurrence predictor for iCCA. |
| Description: |
circ-CCAC1 is an ERBB2-derived circRNA upregulated in CCA tissues and bile/serum EVs. It promotes CCA progression via the miR-514a-5p/YY1/CAMLG axis and exosomal circ-CCAC1 disrupts endothelial barriers and induces angiogenesis by sequestering EZH2 and modulating SH3GL2/ZO-1/Occludin signaling. High circ-CCAC1 expression is associated with poor prognosis, recurrence, adverse clinicopathological features, and diagnostic potential in EVs. |
| Confidence score: |
0.9046 |