| Expression pattern: |
UP |
| Associated gene: |
eIF4A3, Cyclin D1, Cyclin E1, CDK4 |
| Associated microRNA: |
- |
| Biological function: |
Promotes proliferation and migration; promotes cell cycle progression (G0/G1 to S); no significant effect on apoptosis. |
| Molecular mechanism: |
circ_0007766 binds the RBP eIF4A3 and thereby up-regulates cell-cycle genes/proteins Cyclin D1, Cyclin E1 and CDK4. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); cell cycle (promotes); apoptosis (other) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Microarray; Clinical Sample Validation; Transfection; CCK8; Wound Healing Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; RIP (RNA Immunoprecipitation); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ_0007766 was significantly positively correlated with TNM stage and prognosis in lung adenocarcinoma patients. |
| Description: |
circ_0007766 is up-regulated in lung adenocarcinoma tissues and cell lines. Its knockdown suppresses proliferation and migration and induces G0/G1 arrest. Mechanistically, it binds the RBP eIF4A3 and is associated with increased Cyclin D1/Cyclin E1/CDK4 expression to promote cell-cycle progression. |
| Confidence score: |
0.6829 |