| Expression pattern: |
UP |
| Associated gene: |
AAK1, AGO1, JAZF1, NOX4, RRAGD, TBC1D15, ZBTB20 |
| Associated microRNA: |
- |
| Biological function: |
promotes cell invasion and migration; inhibits cell apoptosis; does not affect cell proliferation |
| Molecular mechanism: |
Bioinformatics prediction of miRNA- and protein-binding partners; expression changes of NOX4 and RRAGD after knockdown |
| Biological pathway or process: |
migration (promotes); invasion (promotes); apoptosis (inhibits); proliferation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; divergent primers PCR; Back-Splice Junction PCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); TUNEL; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
hsa_circ_0013290 (circAGL), derived from AGL, is up-regulated in HCC cell lines and mainly localized in the cytoplasm. It promotes invasion and migration and inhibits apoptosis in vitro; knockdown suppresses tumor growth in a cell line-derived xenograft model. Mechanistic work is limited to bioinformatics prediction of binding partners and observed changes in NOX4 and RRAGD after knockdown. |
| Confidence score: |
0.7722 |