| Expression pattern: |
UP |
| Associated gene: |
APC, GSK3beta, beta-catenin |
| Associated microRNA: |
- |
| Biological function: |
Promotes proliferation, migration, invasion, tumorigenesis and metastasis of gastric cancer cells. |
| Molecular mechanism: |
circAXIN1 encodes the protein AXIN1-295aa, which competitively binds APC in the destruction complex, leading to beta-catenin nuclear translocation and activation of Wnt/beta-catenin target gene transcription. |
| Biological pathway or process: |
Wnt/beta-catenin (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Co-IP; Western Blot; IF (Immunofluorescence); ChIP / ChIP-seq; EdU Staining; Transwell Assay; Wound Healing Assay; Colony Formation Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circAXIN1 expression is associated with tumor invasion depth, stage III, lymph node metastasis and poor differentiation; ROC AUC=0.72 for predicting lymph node metastasis; potential prognostic biomarker. |
| Description: |
circAXIN1 is up-regulated in gastric cancer and promotes malignant phenotypes (proliferation, migration, invasion, tumor growth and lung metastasis). It functions mainly by encoding AXIN1-295aa, which competes for APC binding in the Wnt destruction complex, promoting beta-catenin nuclear translocation and activation of Wnt/beta-catenin target genes. |
| Confidence score: |
0.8933 |