| Expression pattern: |
UP |
| Associated gene: |
RAB22A |
| Associated microRNA: |
miR-493-3p |
| Biological function: |
promotes proliferation and stemness; inhibits apoptosis; promotes tumor growth |
| Molecular mechanism: |
ceRNA mechanism: circ_0007385 sponges miR-493-3p to increase RAB22A expression |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); stemness (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; Colony Formation Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
Circ_0007385 is up-regulated in NSCLC tissues/cells and mainly localizes to the cytoplasm. It promotes NSCLC cell proliferation and stemness and inhibits apoptosis, at least in part by sponging miR-493-3p to increase RAB22A expression, and its knockdown suppresses xenograft tumor growth. |
| Confidence score: |
0.734 |