| Expression pattern: |
UP |
| Associated gene: |
GLI1, Hh signaling pathway |
| Associated microRNA: |
miR-616-3p |
| Biological function: |
circ_DCAF6 promotes breast cancer cell proliferation, viability and stemness, and suppresses apoptosis. |
| Molecular mechanism: |
circ_DCAF6 functions as a ceRNA by sponging miR-616-3p, thereby upregulating GLI1 and activating GLI1-dependent Hedgehog signaling. |
| Biological pathway or process: |
Hedgehog (promotes); proliferation (promotes); stemness (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Western Blot; ELISA; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circ_DCAF6 is up-regulated in breast cancer cell lines and promotes breast cancer cell growth and stemness. Mechanistically, cytoplasmic circ_DCAF6 sponges miR-616-3p to increase GLI1 expression and activate the GLI1-dependent Hedgehog pathway. |
| Confidence score: |
0.7909 |