| Expression pattern: |
UP |
| Associated gene: |
eEF2K, p-eEF2, eEF2, cleaved caspase 3, Bax, Bcl-2 |
| Associated microRNA: |
miR-448 |
| Biological function: |
circ-ZEB1 acts as an oncogene in TNBC; circ-ZEB1 knockdown inhibits TNBC cell proliferation and tumor growth, and induces cell apoptosis. |
| Molecular mechanism: |
circ-ZEB1 functions as a ceRNA sponge for miR-448, releasing miR-448-mediated repression of eEF2K and increasing eEF2K expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; TUNEL; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; In Vivo Animal Model |
| Clinical significance: |
The inhibition of circ-ZEB1 could be a potential therapeutic strategy for TNBC. |
| Description: |
circ-ZEB1 is up-regulated in TNBC tissues and cell lines and functions as an oncogenic circRNA. Its knockdown suppresses TNBC cell proliferation and xenograft tumor growth while promoting apoptosis, likely through the circ-ZEB1/miR-448/eEF2K regulatory axis. |
| Confidence score: |
0.7045 |