| Expression pattern: |
DN |
| Associated gene: |
VHL, HIF-1alpha, VEGFA |
| Associated microRNA: |
miR-181c |
| Biological function: |
CircPDE4B inhibits HRECs proliferation, vascular tube formation, VEGFA expression, and retinal pathological angiogenesis in retinopathy of prematurity models. |
| Molecular mechanism: |
CircPDE4B acts as a miR-181c sponge to promote VHL expression, facilitating VHL-mediated ubiquitin degradation of HIF-1alpha and thereby repressing VEGFA expression and pathological angiogenesis. |
| Biological pathway or process: |
angiogenesis (inhibits); proliferation (inhibits); VEGF/VEGFR (inhibits); HIF-1 signaling (inhibits); ubiquitination (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Western Blot; MTT; Tube Formation Assay; Luciferase Reporter Assay; Transfection; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
CircPDE4B might be an effective therapeutic target for retinopathy of prematurity. |
| Description: |
CircPDE4B is downregulated in oxygen-induced retinopathy mouse retina and hypoxia-induced HRECs. Its overexpression suppresses HRECs proliferation, tube formation, VEGFA expression, and pathological angiogenesis by sponging miR-181c to increase VHL and promote ubiquitin-mediated degradation of HIF-1alpha. The study suggests circPDE4B as a potential therapeutic target for retinopathy of prematurity. |
| Confidence score: |
0.5607 |