| Expression pattern: |
UP |
| Associated gene: |
TPT1, AGO2 |
| Associated microRNA: |
miR-1236-3p |
| Biological function: |
Promotes CRC cell proliferation, migration, invasion, metastasis and tumor growth; inhibits autophagy of CRC cells. |
| Molecular mechanism: |
circRNA_103948 acts as a ceRNA/miRNA sponge for miR-1236-3p, relieving suppression of TPT1 and regulating TPT1-mediated autophagy. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); autophagy (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
H
M
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Microarray; FISH / smFISH; ISH (In Situ Hybridization); IHC (Immunohistochemistry); Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Increased circRNA_103948 expression in CRC tissues is correlated with poor prognosis and associated with tumor size. |
| Description: |
circRNA_103948 is upregulated in colorectal cancer tissues and cell lines and predicts poor prognosis. Functionally, it promotes CRC proliferation, migration, invasion, metastasis and xenograft tumor growth while inhibiting autophagy. Mechanistically, circRNA_103948 acts as a miR-1236-3p sponge to relieve repression of TPT1, thereby modulating TPT1-mediated autophagy and CRC progression. |
| Confidence score: |
0.8647 |