| Expression pattern: |
UP |
| Associated gene: |
AGO2 |
| Associated microRNA: |
miR-760 |
| Biological function: |
circPUM1 promotes PCOS cell proliferation and suppresses apoptosis; miR-760 mimic reverses the cellular effects of circPUM1. |
| Molecular mechanism: |
circPUM1 acts as a molecular sponge for miR-760 and decreases miR-760 expression in PCOS cells. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; Annexin V/PI Flow Cytometry |
| Clinical significance: |
circPUM1 may provide a promising therapeutic target for PCOS. |
| Description: |
In this study, circPUM1 was up-regulated in PCOS tissues and insulin-treated granulosa cells/cell lines. circPUM1 promoted PCOS-related granulosa cell viability/proliferation and inhibited apoptosis by sponging miR-760 and decreasing miR-760 expression, suggesting a potential therapeutic target for PCOS. |
| Confidence score: |
0.7158 |