| Expression pattern: |
UP |
| Associated gene: |
LIN28B |
| Associated microRNA: |
miR-384 |
| Biological function: |
Promotes HG-induced endothelial cell dysfunction, including apoptosis, inflammatory responses and oxidative stress; circBPTF knockdown increases cell viability and alleviates these injuries. |
| Molecular mechanism: |
circBPTF functions through a ceRNA-like mechanism by targeting miR-384 and thereby positively regulating LIN28B expression in HG-induced HUVECs. |
| Biological pathway or process: |
ceRNA regulation (promotes); apoptosis (promotes); inflammation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; ELISA; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Potential therapeutic target for diabetes-related vascular complications. |
| Description: |
circBPTF is up-regulated in high glucose-induced HUVEC dysfunction and promotes apoptosis, inflammatory cytokine release and oxidative stress. Mechanistically, circBPTF targets miR-384 to enhance LIN28B expression, while circBPTF knockdown alleviates endothelial injury through the miR-384/LIN28B axis. |
| Confidence score: |
0.7204 |